6I25
Flavin Analogue Sheds Light on Light-Oxygen-Voltage Domain Mechanism
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 64 2 2 |
| 格子定数 [Å] | 106.287, 106.287, 67.859 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 54.620 - 1.970 |
| R因子 | 0.17901 |
| Rwork | 0.177 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a8b |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.697 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 54.620 | 2.020 |
| 分解能 [Å] (高) | 1.970 | 1.970 |
| 独立反射数 | 16503 | |
| <I/σ(I)> | 7.9 | |
| 完全性 [%] | 100.0 | |
| 冗長性 | 20.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5-3 M Na malonate, 0.1 M Tris.acetate |
