6I06
Crystal structure of psychrophilic phosphoglycerate kinase from Pseudomonas TACII18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90005 |
| Spacegroup name | P 32 |
| Unit cell lengths | 59.100, 59.100, 87.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.550 - 2.000 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22890 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6hxe |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.479 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.550 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | |
| Number of reflections | 23306 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 20% PEG 10000 and 0.1 M HEPES at pH 7.5 |
