6HYJ
PSPH Human phosphoserine phosphatase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 2 2 21 |
| 格子定数 [Å] | 48.670, 128.970, 155.710 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 34.230 - 1.929 |
| R因子 | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nnl |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 34.230 | 1.998 |
| 分解能 [Å] (高) | 1.929 | 1.929 |
| Rmerge_l_obs | 0.030 | 0.553 |
| Rmeas | 0.042 | 0.782 |
| Rpim | 0.030 | 0.553 |
| 独立反射数 | 36957 | 3228 |
| <I/σ(I)> | 11.27 | 0.96 |
| 完全性 [%] | 98.7 | 87.57 |
| 冗長性 | 2 | 1.9 |
| CC(1/2) | 0.999 | 0.589 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | CaCl2 0.15 M; sodium cacodylate 0.1 M pH 6.5; PEG 2000 20% |
