6HFX
Crystal structure of Extracellular Domain 1 (ECD1) of FtsX from S. pneumonie in complex with n-decyl-B-D-maltoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979257 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.496, 75.496, 95.311 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.576 - 2.160 |
R-factor | 0.2626 |
Rwork | 0.260 |
R-free | 0.31570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6he6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.169 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.580 | 46.580 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.072 | 1.526 |
Rmeas | 0.074 | 1.563 |
Rpim | 0.016 | 0.332 |
Number of reflections | 28508 | |
<I/σ(I)> | 23.1 | |
Completeness [%] | 98.8 | |
Redundancy | 16.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291.15 | 0.1 M Sodium Citrate pH=5.6, 0.2 M Potassium-Sodium Tartrate and 2M Amonium Sulfate |