6H8K
Crystal structure of a variant (Q133C in PSST) of Yarrowia lipolytica complex I
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 316.310, 316.310, 819.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.790 |
| R-factor | 0.361 |
| Rwork | 0.361 |
| R-free | 0.36300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wz7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.990 |
| High resolution limit [Å] | 3.790 | 3.790 |
| Rmerge | 0.204 | |
| Number of reflections | 120019 | |
| <I/σ(I)> | 13.2 | 0.7 |
| Completeness [%] | 99.6 | |
| Redundancy | 21 | |
| CC(1/2) | 1.000 | 0.439 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | PEG 3350, calcium acetate, glycerol |
