6H7A

Structure of Leishmania PABP1 (domain J).

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9760
Spacegroup name	P 65
格子定数 [Å]	82.970, 82.970, 46.080
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	27.158 - 2.030
R因子	0.2027
Rwork	0.201
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pth
結合長の平均二乗偏差(RMSD) [Å]	0.002
結合角の平均二乗偏差(RMSD) [度]	0.423
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000	2.103
分解能 [Å] (高)	2.030	2.030
Rmerge_l_obs	0.128	1.821
Rmeas	0.132	1.868
Rpim	0.030	0.419
独立反射数	11824	1175
<I/σ(I)>	14.98
完全性 [%]	99.8	99.91
冗長性	19.9	19.8
CC(1/2)	0.999	0.683

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium sulphate, 0.1 M Sodium cacodylate at pH 6.5, 30% PEG8000