6H1E

Crystal structure of C21orf127-TRMT112 in complex with SAH and H4 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 42 21 2
Unit cell lengths	71.231, 71.231, 130.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 1.900
R-factor	0.1983
Rwork	0.196
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h1d
RMSD bond length	0.007
RMSD bond angle	0.917
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.180	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.187	2.740
Rpim	0.056	0.857
Number of reflections	27384
<I/σ(I)>	1.2
Completeness [%]	100.0	99.89
Redundancy	22.5	21.9
CC(1/2)	0.999	0.296

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.5, 1.1-1.3 M Sodium Citrate