6GWK
The crystal structure of Hfq from Caulobacter crescentus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 43 |
Unit cell lengths | 97.322, 97.322, 203.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.810 - 2.150 |
R-factor | 0.2797 |
Rwork | 0.278 |
R-free | 0.30510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pno |
RMSD bond length | 0.003 |
RMSD bond angle | 0.520 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3112) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.810 | 2.227 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.123 | 0.628 |
Rmeas | 0.155 | 0.823 |
Rpim | 0.092 | 0.525 |
Number of reflections | 100169 | 10091 |
<I/σ(I)> | 9.44 | 0.46 |
Completeness [%] | 97.4 | 99.06 |
Redundancy | 2 | 1.9 |
CC(1/2) | 0.990 | 0.618 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2M sodium thiocyanate, 17 % PEG 3350 |