6GMU

Serum paraoxonase-1 by directed evolution with the L69G/H134R/F222S/T332S mutations

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9724
Spacegroup name	P 43 21 2
格子定数 [Å]	94.318, 94.318, 142.410
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	33.308 - 2.700
R因子	0.1839
Rwork	0.179
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3srg
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	0.899
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.750
分解能 [Å] (高)	2.700	2.700
Rmerge_l_obs	0.157	0.853
Rmeas	0.163	0.886
Rpim	0.045	0.238
独立反射数	18335	881
<I/σ(I)>	16.53	2.96
完全性 [%]	99.9	100
冗長性	13.5	13.7
CC(1/2)		0.927

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Bis-Tris Propane pH 6.5, 0.2M Sodium Bromide and 20% PEG 3350