6GJQ
human NBD1 of CFTR in complex with nanobody T27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979490 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.490, 118.150, 180.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.930 - 2.490 |
R-factor | 0.2005 |
Rwork | 0.199 |
R-free | 0.23960 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.560 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.182 | 0.640 |
Rmeas | 0.193 | 1.741 |
Number of reflections | 48671 | |
<I/σ(I)> | 10.35 | |
Completeness [%] | 99.2 | |
Redundancy | 9.15 | |
CC(1/2) | 0.995 | 0.467 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350 |