6GG7

cyanobacterial GAPDH with full-length CP12

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96861
Spacegroup name	P 43 21 2
格子定数 [Å]	141.052, 141.052, 75.931
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	60.416 - 1.320
R因子	0.1468
Rwork	0.145
R-free	0.17350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4boy
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	0.921
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	60.420	1.367
分解能 [Å] (高)	1.320	1.320
Rmerge_l_obs	0.127	0.918
Rmeas	0.132	0.957
独立反射数	178371	17624
<I/σ(I)>	11.43
完全性 [%]	100.0	100
冗長性	12.8	12.6
CC(1/2)	0.988	0.586

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION		290	Tryptone CM1(A6) 1% Tryptone, 25% PEG, 100 mM Hepes pH 8.