6GG7

cyanobacterial GAPDH with full-length CP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96861
Spacegroup name	P 43 21 2
Unit cell lengths	141.052, 141.052, 75.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.416 - 1.320
R-factor	0.1468
Rwork	0.145
R-free	0.17350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4boy
RMSD bond length	0.005
RMSD bond angle	0.921
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.420	1.367
High resolution limit [Å]	1.320	1.320
Rmerge	0.127	0.918
Rmeas	0.132	0.957
Number of reflections	178371	17624
<I/σ(I)>	11.43
Completeness [%]	100.0	100
Redundancy	12.8	12.6
CC(1/2)	0.988	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		290	Tryptone CM1(A6) 1% Tryptone, 25% PEG, 100 mM Hepes pH 8.