6GFP
cyanobacterial GAPDH with NADP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 141.469, 141.469, 74.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.737 - 1.540 |
R-factor | 0.1649 |
Rwork | 0.164 |
R-free | 0.18330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zdf |
RMSD bond length | 0.006 |
RMSD bond angle | 0.890 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.740 | 1.595 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.096 | 0.889 |
Rmeas | 0.100 | |
Rpim | 0.028 | 0.264 |
Number of reflections | 111368 | 10960 |
<I/σ(I)> | 13.92 | 1.94 |
Completeness [%] | 100.0 | 99.95 |
Redundancy | 12.6 | 12.2 |
CC(1/2) | 0.999 | 0.567 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 100 mM Hepes pH7.0 16% PEG3350 4% Tacsimate 5 mM DL-Glyceraldehyde-3-phosphate 5 mM NADP+, 5 mM NADPH 5 mM 3-phosphoglycerate 1 unit phosphoglycerate kinase |