6GF8

Molecular basis of egg coat filament cross-linking: structure of the glycosylated ZP1 ZP-N1 domain homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	90.774, 125.734, 105.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.725 - 3.100
R-factor	0.2127
Rwork	0.210
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Monomer 6GF6
RMSD bond length	0.004
RMSD bond angle	0.657
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.740	3.240
High resolution limit [Å]	3.100	3.100
Rmeas	0.093	1.114
Rpim	0.043	0.520
Number of reflections	11119	1328
<I/σ(I)>	9.5	1
Completeness [%]	99.1	96.9
Redundancy	4.1	4.2
CC(1/2)	1.000	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	29% (V/V) MPD, 0.1 M MES PH 6.5