6GF6
Molecular basis of egg coat filament cross-linking: high-resolution structure of the partially deglycosylated ZP1 ZP-N1 domain homodimer
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.7710 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 75.980, 75.980, 101.880 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 36.966 - 2.300 |
| R因子 | 0.1958 |
| Rwork | 0.192 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Partially refined model derived from SAD phasing of a gold derivative |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.738 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3409: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 37.990 | 2.400 |
| 分解能 [Å] (高) | 2.300 | 2.300 |
| Rmeas | 0.116 | 1.498 |
| Rpim | 0.026 | 0.495 |
| 独立反射数 | 13550 | 1422 |
| <I/σ(I)> | 22.2 | 1.4 |
| 完全性 [%] | 98.0 | 84.6 |
| 冗長性 | 19.7 | 8.3 |
| CC(1/2) | 1.000 | 0.550 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.6 M LiCl, 0.1 M tri-sodium citrate pH 5.5 |
