6G8L
14-3-3sigma in complex with a L132beta3L mutated YAP pS127 phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.258, 111.969, 62.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.533 - 1.370 |
| R-factor | 0.1362 |
| Rwork | 0.136 |
| R-free | 0.15720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mhr |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.290 | 66.290 | 1.390 |
| High resolution limit [Å] | 1.370 | 7.500 | 1.370 |
| Rmerge | 0.133 | 0.118 | 0.640 |
| Rmeas | 0.140 | 0.124 | 0.723 |
| Rpim | 0.043 | 0.036 | 0.327 |
| Total number of observations | 602191 | ||
| Number of reflections | 60861 | 438 | 2881 |
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 99.8 | 99.9 | 96 |
| Redundancy | 9.9 | 11.4 | 4.4 |
| CC(1/2) | 0.992 | 0.979 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.095 M HEPES, pH 7.1, 29% PEG 400, 0.19 M CaCl2, 5% glycerol |
