6G76

Phosphorylated RSK4 N-terminal Kinase Domain in complex with AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	62.489, 52.331, 115.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.667 - 3.000
R-factor	0.226
Rwork	0.222
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g77
RMSD bond length	0.003
RMSD bond angle	0.673
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.670	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.045	0.157
Rmeas	0.064
Number of reflections	15236	1511
<I/σ(I)>	7.67
Completeness [%]	99.8	99.93
Redundancy	2	2
CC(1/2)	0.995	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	18 % PEG 6000, 0.1 M TRIS pH8, 2 mM Zinc chloride