6G20
Crystal structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in its functional Meta-F intermediate state.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9724 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 183.114, 183.114, 179.655 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 158.580 - 2.160 |
R-factor | 0.2102 |
Rwork | 0.209 |
R-free | 0.23225 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6g1z |
RMSD bond length | 0.011 |
RMSD bond angle | 1.468 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.790 | 2.280 |
High resolution limit [Å] | 2.160 | 2.160 |
Rpim | 0.031 | 0.409 |
Number of reflections | 95000 | 13679 |
<I/σ(I)> | 18.3 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 | 20 |
CC(1/2) | 0.999 | 0.672 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 279 | (NH4)2SO4, VAPOR DIFFUSION, TEMPERATURE 279K |