6FZG
PPAR gamma mutant complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 60.375, 60.375, 162.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.426 - 2.100 |
R-factor | 0.1658 |
Rwork | 0.164 |
R-free | 0.20840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1prg |
RMSD bond length | 0.006 |
RMSD bond angle | 0.820 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.430 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.079 | 0.542 |
Number of reflections | 18330 | |
<I/σ(I)> | 18.02 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 25% PEG 3350, 0.2 M NaCl, 0.1 M Hepes pH 7.5 |