6FYX
Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Number of Particle | 706713 |
| Software | RELION,REFMAC,EMAN,EPU,Gctf,UCSF Chimera,Coot |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
