6FPT
Crystal structure of Danio rerio Lin41 filamin-NHL domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.800, 90.590, 131.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.430 - 2.600 |
| R-factor | 0.1847 |
| Rwork | 0.181 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology models based on PDB IDs 4UMG and 1Q7F |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 42647 | 3006 |
| <I/σ(I)> | 5.28 | |
| Completeness [%] | 91.6 | 86.8 |
| Redundancy | 1.9 | 1.8 |
| CC(1/2) | 0.987 | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% PEG 8000 0.2 M magnesium chloride 0.1 M Tris pH 7.0 0.1 M trisodium citrate |
