6FEZ
Ryegrass mottle virus protease domain
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 37.110, 115.990, 57.920 |
| 格子定数 [度] | 90.00, 105.27, 90.00 |
精密化法
| 残基 | 57.990 - 2.300 |
| R因子 | 0.23229 |
| Rwork | 0.229 |
| R-free | 0.29018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyo |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.767 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 57.990 | 2.420 |
| 分解能 [Å] (高) | 2.300 | 2.300 |
| Rmerge_l_obs | 0.123 | 0.548 |
| Rmeas | 0.149 | 0.662 |
| Rpim | 0.083 | 0.365 |
| 独立反射数 | 19160 | 2981 |
| <I/σ(I)> | 5.3 | 2.1 |
| 完全性 [%] | 96.0 | 98.69 |
| 冗長性 | 3 | 3.1 |
| CC(1/2) | 0.989 | 0.644 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES 7.5, 22.5% PEG 3350, 0.22M LiSO4, 3% DMSO |
