6EK4

PaxB from Photorhabdus luminescens

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
格子定数 [Å]	104.590, 70.180, 136.890
格子定数 [度]	90.00, 108.41, 90.00

精密化法

残基	15.000 - 2.800
R因子	0.24205
Rwork	0.240
R-free	0.26835
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Semet derivative of PaxB
結合長の平均二乗偏差(RMSD) [Å]	0.004
結合角の平均二乗偏差(RMSD) [度]	0.696
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000	2.900
分解能 [Å] (高)	2.800	2.800
Rmeas	0.069	0.515
独立反射数	44675
<I/σ(I)>	9.7	1.8
完全性 [%]	95.3	94.7
冗長性	2.9
CC(1/2)	0.997	0.729

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2 M MgCl2; 0.1 M Bis-Tris, 25% PEG 3350; 0.2M NaSCN