6EER

Structure of glycine-bound GoxA from Pseudoalteromonas luteoviolacea

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
格子定数 [Å]	110.242, 93.464, 187.903
格子定数 [度]	90.00, 95.02, 90.00

精密化法

残基	48.295 - 1.820
R因子	0.1774
Rwork	0.175
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6byw
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.885
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	48.300	1.850
分解能 [Å] (高)	1.820	1.820
Rmerge_l_obs	0.068	0.405
Rpim	0.062	0.365
独立反射数	338105	16759
<I/σ(I)>	10
完全性 [%]	99.5	99.9
冗長性	3.7	3.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	BATCH MODE	7.5	292	22% w/v PEG 3350, 0.1 M ammonium sulfate, 0.1 M HEPES pH 7.5 mixed 1:1 with protein at 10 mg/mL