6ECA
Lactobacillus rhamnosus Beta-glucuronidase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 154.800, 154.800, 241.906 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 29.497 - 2.853 |
| R因子 | 0.1522 |
| Rwork | 0.149 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lpg |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.921 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 29.500 | 2.970 |
| 分解能 [Å] (高) | 2.850 | 2.850 |
| 独立反射数 | 374145 | |
| <I/σ(I)> | 12.6 | |
| 完全性 [%] | 99.2 | |
| 冗長性 | 9.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293.15 | 17% PEG3350, 0.4 M NaCl, and and 0.1 Tris HCl pH 7.4 |
