6E4W
Structure of AMPK bound to activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 123.970, 123.970, 402.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.580 - 3.350 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.24400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | autoXDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.580 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.122 | 0.689 |
| Number of reflections | 27326 | |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 750 MM AMMONIUM SULFATE, 500 MM LITHIUM SULFATE, 100 MM TRISODIUM CITRATE, 1% ETHYLENE GLYCOL |
