6E1F
Crystal structure of the SWIRM domain of human histone lysine-specific demethylase LSD1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 49.957, 57.514, 97.019 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 35.180 - 1.160 |
| R因子 | 0.133 |
| Rwork | 0.133 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5it3 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.907 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0230) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 35.180 | 1.190 |
| 分解能 [Å] (高) | 1.160 | 1.160 |
| 独立反射数 | 94426 | |
| <I/σ(I)> | 50.7 | 3.5 |
| 完全性 [%] | 96.8 | 86.4 |
| 冗長性 | 11.4 | 8.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris, pH 6.5, 0.1 M potassium chloride, 1.5 M ammonium sulfate |
