6E1C

Crystal structure of a MauG-like protein associated with microbial copper homeostasis

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.722003
Spacegroup name	P 21 21 21
格子定数 [Å]	71.557, 85.757, 133.600
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	42.879 - 2.617
R因子	0.2046
Rwork	0.198
R-free	0.27810
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.664
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	42.880	2.690
分解能 [Å] (高)	2.617	2.617
Rmerge_l_obs	0.148	0.846
Rmeas	0.152	0.871
Rpim	0.035	0.202
独立反射数	24860
<I/σ(I)>	21.26
完全性 [%]	97.6
冗長性	10.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		295	PPG 400, Bis-Tris