6DO2
Crystal structure of human GRP78 in complex with 7-deaza-2'-C-methyladenosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.62 - 2.5 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.410, 75.020, 89.480 |
Unit cell angles | 90.00, 99.11, 90.00 |
Refinement procedure
Resolution | 24.890 - 1.700 |
R-factor | 0.208 |
Rwork | 0.207 |
R-free | 0.23200 |
Data reduction software | XDS |
Phasing software | BUSTER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 79463 | |
<I/σ(I)> | 15.72 | |
Completeness [%] | 99.3 | |
Redundancy | 4.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 24 to 26% PEG 3350, 0.1 M Tris-HCl and 0.2 M NaCl (by microseeding with the initial crystals from 0.1 M Tris- HCl pH 8.5, 25% PEG 3350 and 0.1 M Na/K tartrate) |