6DO2

Crystal structure of human GRP78 in complex with 7-deaza-2'-C-methyladenosine

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.62 - 2.5
Spacegroup name	P 1 21 1
格子定数 [Å]	55.410, 75.020, 89.480
格子定数 [度]	90.00, 99.11, 90.00

精密化法

残基	24.890 - 1.700
R因子	0.208
Rwork	0.207
R-free	0.23200
Data reduction software	XDS
Phasing software	BUSTER
Refinement software	BUSTER (2.10.2)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.800
分解能 [Å] (高)	1.700	1.700
独立反射数	79463
<I/σ(I)>	15.72
完全性 [%]	99.3
冗長性	4.45

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		298	24 to 26% PEG 3350, 0.1 M Tris-HCl and 0.2 M NaCl (by microseeding with the initial crystals from 0.1 M Tris- HCl pH 8.5, 25% PEG 3350 and 0.1 M Na/K tartrate)