6DF9

Kaiso (ZBTB33) E535Q zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 2
格子定数 [Å]	44.452, 183.142, 105.683
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	43.198 - 2.319
R因子	0.1767
Rwork	0.172
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.015
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	43.198	2.400
分解能 [Å] (高)	2.319	2.320
Rmerge_l_obs	0.061
Rmeas	0.068
独立反射数	18818	1559
<I/σ(I)>	23.21
完全性 [%]	97.7	82.53
冗長性	11.5	8.4
CC(1/2)	0.997	0.974

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.2 M Disodium Tartrate, 20 % PEG 3350