6D9L
Ternary RsAgo Complex with Guide RNA and Target DNA Containing G-A Non-canonical Pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.045, 118.253, 117.827 |
Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
Resolution | 41.670 - 2.600 |
R-factor | 0.2138 |
Rwork | 0.212 |
R-free | 0.25330 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.972 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.109 | 0.777 |
Number of reflections | 55738 | |
<I/σ(I)> | 11.1 | 2.3 |
Completeness [%] | 98.1 | 97.8 |
Redundancy | 3.5 | 3.4 |
CC(1/2) | 0.990 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7 |