6D49
Cell Surface Receptor in Complex with Ligand at 1.80-A Resolution
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 29.369, 68.765, 73.468 |
| 格子定数 [度] | 90.00, 96.97, 90.00 |
精密化法
| 残基 | 36.462 - 1.801 |
| R因子 | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6d48 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.154 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1-2575_1496) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 36.462 | 1.840 |
| 分解能 [Å] (高) | 1.800 | 1.800 |
| Rmerge_l_obs | 0.073 | 0.599 |
| Rmeas | 0.081 | 0.664 |
| Rpim | 0.035 | 0.284 |
| 独立反射数 | 26760 | 1534 |
| <I/σ(I)> | 12.6 | 2.3 |
| 完全性 [%] | 99.4 | 94.8 |
| 冗長性 | 5.3 | 5.2 |
| CC(1/2) | 0.999 | 0.877 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5 0.6 M NaCl 18% PEG 4000 |
