6CT7
Fab of anti-a-synuclein antibody BIIB054 in complex with acetylated a-synuclein peptide (1-10)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.849, 101.492, 73.400 |
Unit cell angles | 90.00, 117.30, 90.00 |
Refinement procedure
Resolution | 24.860 - 1.903 |
R-factor | 0.1819 |
Rwork | 0.180 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.953 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.972 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.085 | |
Number of reflections | 72048 | |
<I/σ(I)> | 5.3 | |
Completeness [%] | 97.0 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 277 | 18% w/v PEG 3350, 17% w/v PEG 400, 4.8% v/v 2-propanol, and 100 mM CAPSO pH 8.9 |