6CDO
Structure of vaccine-elicited HIV-1 neutralizing antibody vFP16.02 in complex with HIV-1 fusion peptide residue 512-519
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 141.027, 141.027, 77.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.954 - 2.099 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.22640 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.108 | 0.051 | 0.931 |
| Rmeas | 0.112 | 0.053 | 0.978 |
| Rpim | 0.030 | 0.014 | 0.294 |
| Total number of observations | 641724 | ||
| Number of reflections | 46197 | 2535 | 2251 |
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 100.0 | 99.6 | 99.7 |
| Redundancy | 13.9 | 13.4 | 9.7 |
| CC(1/2) | 0.999 | 0.858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M NaoAc pH 4.5, 2 M AmSO4 |
