6BL6

Crystallization of lipid A transporter MsbA from Salmonella typhimurium

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	80
Detector technology	CCD
Collection date	2016-01-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.127085
Spacegroup name	P 21 21 2
格子定数 [Å]	82.052, 154.819, 228.886
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	50.010 - 2.800
R因子	0.27104
Rwork	0.270
R-free	0.30071
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b5w
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.368
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.010	2.850
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs	0.136	0.136
Rmeas	0.148
Rpim	0.057	0.057
独立反射数	60504
<I/σ(I)>	10
完全性 [%]	84.9	82
冗長性	5	4.1
CC(1/2)	0.893

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	MsbA purified in detergent micelles (UDM/FA231) is mixed with lipid A prior to crystallization and mixed 1: 1 with well solution 20 mM Tris-Hcl (pH 7.5) and 15-20 5 PEG 300 . Crystals appeared in month (approx.)