6B6A
Beta-Lactamase, 2secs timepoint, mixed, shards crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2016-09-09 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.850, 95.310, 111.870 |
| Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
| Resolution | 43.412 - 2.298 |
| R-factor | 0.2421 |
| Rwork | 0.241 |
| R-free | 0.26670 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.709 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 65076 | |
| <I/σ(I)> | 5.4 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 329.82 | 59 |
| CC(1/2) | 0.354 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 300 | Ammonium phosphate |
