6AKM

Crystal structure of SLMAP-SIKE1 complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 32 2 1
格子定数 [Å]	66.763, 66.763, 61.353
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	42.080 - 2.300
R因子	0.2074
Rwork	0.204
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6akk
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	0.886
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.380
分解能 [Å] (高)	2.300	2.300
Rmerge_l_obs	0.094	0.821
Rmeas	0.097	0.844
Rpim	0.022	0.193
独立反射数	7336	713
<I/σ(I)>	21.6	5.2
完全性 [%]	100.0	100
冗長性	18.8	19
CC(1/2)		0.948

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M sodium citrate, pH 5.5; 15% (w/v) PEG 6000