6AB8
Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with ZLys
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 102.134, 43.893, 62.070 |
| 格子定数 [度] | 90.00, 98.83, 90.00 |
精密化法
| 残基 | 42.283 - 1.753 |
| R因子 | 0.1752 |
| Rwork | 0.172 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zim |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.149 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.790 |
| 分解能 [Å] (高) | 1.750 | 1.750 |
| Rmeas | 0.089 | |
| Rpim | 0.046 | 0.352 |
| 独立反射数 | 27397 | 1346 |
| <I/σ(I)> | 19.1 | |
| 完全性 [%] | 99.6 | |
| 冗長性 | 3.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | Tris-HCl (pH 8.5), PEG 200, KCl, MgCl2, sodium citrate |
