6VYO

Crystal structure of RNA binding domain of nucleocapsid phosphoprotein from SARS coronavirus 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-25
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	66.238, 77.430, 114.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.050 - 1.700
R-factor	0.1615
Rwork	0.159
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2OFZ.pdb
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.131	0.070	1.134
Rmeas	0.137	0.073	1.192
Rpim	0.038	0.020	0.357
Total number of observations	798795
Number of reflections	64592	3480	3101
<I/σ(I)>	6.9
Completeness [%]	99.7	99.6	97.3
Redundancy	12.4	12.6	10.2
CC(1/2)		0.997	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	20.0% PEG6000, 0.1M MES, 10.0 mM Zinc chloride