6OAK

Structure of human PARG complexed with JA2131

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	66.497, 89.333, 94.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.078 - 1.700
R-factor	0.1835
Rwork	0.182
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b1g
RMSD bond length	0.004
RMSD bond angle	0.799
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.078	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.139	2.688
Number of reflections	62296	6098
<I/σ(I)>	17.71
Completeness [%]	100.0	99
Redundancy	19.1	13.1
CC(1/2)	0.999	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M PCTP, pH 7.5, 0.2 M (NH3)2SO4, 17% PEG3350