6NYO

Crystal structure of a human Cdc34-ubiquitin thioester mimetic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	108
Detector technology	CCD
Collection date	2017-02-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	44.585, 55.737, 119.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.784 - 1.502
R-factor	0.1759
Rwork	0.175
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mdk
RMSD bond length	0.006
RMSD bond angle	0.786
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.069	0.976
Rpim	0.032	0.464
Number of reflections	48439
<I/σ(I)>	17.5
Completeness [%]	99.9
Redundancy	5.67
CC(1/2)	0.997	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.03 M potassium dihydrogen phosphate, 23% PEG 8,000