6LYJ

The crystal structure of SAUGI/EBVUDG complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	P 21 21 21
Unit cell lengths	49.480, 93.496, 156.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.100
R-factor	0.1752
Rwork	0.173
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ays
RMSD bond length	0.003
RMSD bond angle	0.677
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.079	0.356
Number of reflections	41768	3865
<I/σ(I)>	27.7
Completeness [%]	96.5
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	TrisHCl, PEG 6000