6JBR

Tps1/UDP/T6P complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	99.279, 172.493, 141.703
Unit cell angles	90.00, 90.92, 90.00

Refinement procedure

Resolution	29.798 - 2.030
R-factor	0.212
Rwork	0.211
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uqt
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.030	2.030
Rmerge	0.057	0.451
Rpim	0.023	0.195
Number of reflections	305744	30304
<I/σ(I)>	32.26
Completeness [%]	99.6
Redundancy	3.5
CC(1/2)	1.000	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	289	0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, 20% PEG 3350 (v/v)