6GB3
A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - M49S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.879, 30.978, 49.477 |
| Unit cell angles | 90.00, 112.90, 90.00 |
Refinement procedure
| Resolution | 45.579 - 1.752 |
| R-factor | 0.1558 |
| Rwork | 0.153 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kuk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.580 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.115 | 0.187 |
| Rmeas | 0.125 | 0.262 |
| Number of reflections | 12224 | 601 |
| <I/σ(I)> | 18.9 | 6.9 |
| Completeness [%] | 94.9 | 87.1 |
| Redundancy | 3.1 | 1.7 |
| CC(1/2) | 0.997 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292.15 | 0.1 M Sodium citrate, 1 M Ammonium dihydrogen phosphate |
