5ZXH
The structure of MT189-tubulin complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 104.910, 157.060, 182.838 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.800 |
| R因子 | 0.21066 |
| Rwork | 0.208 |
| R-free | 0.25899 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.850 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| 独立反射数 | 74342 | |
| <I/σ(I)> | 23.3 | |
| 完全性 [%] | 99.9 | |
| 冗長性 | 7.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 6% POLY(ETHYLENE GLYCOL) 4000, 8% GLYCEROL, 0.1 M MES, 30 MM CACL2, 30 MM MGCL2 (pH 6.7) |
