5ZWY
Ribokinase from Leishmania donovani
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 61 |
| 格子定数 [Å] | 100.253, 100.253, 126.771 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 46.610 - 1.950 |
| R因子 | 0.18438 |
| Rwork | 0.183 |
| R-free | 0.21781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv7 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.340 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 46.610 | 2.000 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| Rmerge_l_obs | 0.101 | 0.604 |
| 独立反射数 | 52575 | 27813 |
| <I/σ(I)> | 0.101 | |
| 完全性 [%] | 100.0 | |
| 冗長性 | 7.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 296 | Citric acid, NaCl, Glycerol |
