5ZWP
Crystal structure of the delta-class glutathione transferase from Musca domestica
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 51.260, 90.909, 51.418 |
| 格子定数 [度] | 90.00, 107.56, 90.00 |
精密化法
| 残基 | 49.030 - 1.400 |
| R因子 | 0.15773 |
| Rwork | 0.157 |
| R-free | 0.17605 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jlv |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.912 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 49.030 | 1.480 |
| 分解能 [Å] (高) | 1.400 | 1.400 |
| Rmerge_l_obs | 0.045 | 0.115 |
| 独立反射数 | 84246 | 11834 |
| <I/σ(I)> | 8.2 | 5.9 |
| 完全性 [%] | 95.9 | 95.9 |
| 冗長性 | 1.9 | 1.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M sodium citrate, pH 5.6, 1.8 M ammonium sulfate, 0.1 M tartrate |
