5ZVQ
Crystal structure of recombination mediator protein RecR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.5417 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 35.920, 100.220, 136.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.680 - 2.500 |
| R-factor | 0.2384 |
| Rwork | 0.236 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vdd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.114 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.680 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 8602 | |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 96.8 | |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 295 | 0.2 M Potassium chloride, 0.05 M HEPES pH 7.5, 35% v/v Pentaerythritol propoxylate (5/4 PO/OH) |
