5ZO6
Crystal structure of C166, a backbone circularized G-CSF
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 47.180, 48.050, 54.960 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 36.170 - 1.700 |
| R因子 | 0.16972 |
| Rwork | 0.167 |
| R-free | 0.21672 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bgc |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.019 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.884 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 47.150 | 1.730 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.060 | 0.271 |
| 独立反射数 | 14235 | 747 |
| <I/σ(I)> | 21.8 | 7.3 |
| 完全性 [%] | 99.7 | 99.5 |
| 冗長性 | 6.9 | 7.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | sodium acetate trihydrate, sodium chloride dehydrate, PEG 1500, MPD |
